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Image Search Results
Journal: Journal of Biological Chemistry
Article Title: Regulation of Gene Transcription by Voltage-gated L-type Calcium Channel, Cav1.3
doi: 10.1074/jbc.m114.586883
Figure Lengend Snippet: FIGURE 3. The C terminus of the Cav1.3 channel is localized in the nucleus of cardiac myocytes from wild-type mice. a, immunofluorescence confocal microscopic images of neonatal mouse cardiomyocytes stained using an antibody recognizing an epitope in the II-III cytoplasmic loop of the Cav1.3 channel (amino acids 859–875, loop antibody, upper panels). No detectable nuclear fluorescence is observed. In contrast, a high nuclear fluorescence signal is detected when an antibody that recognizes the C terminus of Cav1.3 (amino acids 1661–1990) is used (lower panels). b, isolated nuclei from adult atrial myocytes. The C-terminal antibody of the Cav1.3 channel detects a high nuclear fluorescence in contrast to the antibody directed against the II-III cytoplasmic loop. Nuclei were stained using DAPI (blue). c, adult atrial myo- cytes co-stained with anti-Cav1.3 (loop antibody, red) and -actinin2 (green) antibodies. Scale bars are 10 m. Right panel shows the merged image at higher magnification. d, isolated nuclei from adult atrial myocytes. Two dif- ferent monoclonal antibodies for the Cav1.3 Ca2 channel were used includ- ing: 1) anti-Cav1.3 Ca2 channel (NeuroMab clone L48A/9) directed against amino acids 859–875 in the N terminus of rat Cav1.3, and 2) anti-Cav1.3 Ca2 channel (NeuroMab clone N38/8) directed against amino acids 2025–2161 in
Article Snippet: 9) Monoclonal
Techniques: Immunofluorescence, Staining, Fluorescence, Isolation, Bioprocessing